Cultural and Biochemical characteristics of Staphylococcus aureus

 

Cultural characteristics of Staphylococcus aureus

1. Staphylococci grow readily on most bacteriologic media under aerobic or microaerophilic conditions.
2. Colonies on solid media are round, smooth, raised, and glistening.
3. S. aureus usually forms gray to deep golden yellow colonies.
4. Mannitol Salt Agar: circular, 2–3 mm in diameter, with a smooth, shiny surface; colonies appear opaque and are often pigmented, golden yellow. 

5. Tryptic Soy Agar: circular, convex and entire margin. 

6. Blood Agar: beta-hemolysis.

1. Brain heart infusion agar: Yellow pigmented colonies.

Biochemical characteristics of Staphylococcus aureus

• Catalase positive
• Oxidase negative
• OF test – fermentative
• Coagulase positive: presence of free and /or bound coagulase
• Indole negative
• Gas negative
• Hydrogen sulphide negative
• Methyl red positive
• VP positive
• Nitrate reduction positive
• Gelatin hydrolysis positive
• Beta hemolysis on Blood agar
• Citrate positive
• Motility negative
• PYR negative
• Urease positive

Cultural and Biochemical characteristics of Staphylococcus aureus

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Biochemical Characteristics, Biochemical characteristics of Staphylococcus aureus, Cultural Characteristics, Cultural characteristics of Staphylococcus aureus, Staphylococcus, Staphylococcus aureus