Laboratory diagnosis of Streptococcus pyogenes
1. Specimens
- Throat swab
- Pus
- Cerebrospinal fluid
- Blood
- Serum for antibody determinant
2. Smear
- Gram staining
- Purple color cocci in chain arrangement
- Not to be confused with Viridans Streptococci from throat swab sample since both have same appreance
Culture
- Culture on blood agar
- Addition of bacitracin in inoculum: S pyogenes are sensitive to bacitracin
- Colonial appearance: Grayish white, transparent to translucent, matte or glossy; smooth; flat;large zone of beta hemolysis
- Catalase negative, oxidase negative and PYR positive
4. Antigen detection tests
- Enzyme immunoassay (EIA)
- Agglutination test
- Kits uses enzymatic or chemical method to extract antigen from throat swab and demonstrate the presence of antigen using EIA or agglutination test (visible clumping)
- More sensitive assays are DNA probes and Nucleic acid amplification techniques
5. Serologic tests
- Detection of antibody titer after 3 to 4 weeks after exposure to organism
- Antibodies include ASO, anti-DNase B, anti- hyaluronidase, antistreptokinase, anti- M type specific antibodies
- Anti Streptolysin O (ASO) is most widely used.
Treatment of Streptococcus pyogenes
- S pyogenes are susceptible to penicillin (benzylpenicillin (penicillin G) or oral phenoxymethylpenicillin (penicillinV)
- For penicillin allergic patients, erythromycin is a drug of choice
- In some cases clindamycin or vancomycin is also recommended
Prevention and control of Streptococcus pyogenes
- Maintenance of personal hygiene
- Chemoprophylaxis: prophylactic use of antibiotics in some streptococcal infections: rheumatic fever,
Laboratory diagnosis of Streptococcus pyogenes
Tags:
Control, Laboratory diagnosis, Laboratory diagnosis of Streptococcus pyogenes, Prevention, Prevention and control of Streptococcus pyogenes, Streptococcus, Streptococcus pyogenes, Treatment, Treatment of Streptococcus pyogenes
1 Comments
I just want to share this here,
ReplyDeleteI was diagnosed with stage 3 breast cancer in August 2010. A valuable friend told me about Dr. Itua Herbal Center in West Africa. She gave me her phone number and email address. I quickly contacted him to guarantee that his herbal medicines will heal my cancer and I will heal forever I said OK.I ask him what is the healing process, he asks me to pay the fees I did and within 7 working days he sent me the herbal medicine and then he asked me I told my friend Gomez about the herbal drug so that he gave me to go and drink it.So after drinking for two weeks, I was cured, I am so grateful and I promise that I will do it I recommend to anyone who has cancer and that that I am doing. Herbal medicine Dr. Itua makes me believe that there is hope for people with all kinds of disease or need herbal medicine for illness , Here is his contact information [Email ... drituaherbalcenter@gmail.com. Web: www.drituaherbalcenter.com.
Thank you once again blog admin.